Jasmonates disrupt carrageenan synthesis during carposporogenesis in the red seaweed Grateloupia imbricata

In this study, we monitored changes in cell wall sulfated galactans the red seaweed Grateloupia imbricata after rapid (48 h) induction of cystocarp maturation and carpospore development by volatile hormone methyl jasmonate (MEJA). Synthesis galactans, carrageenans, which requires UDP galactose, was followed expression analysis genes encoding phosphoglucomutase (PGM), galactose-1-phosphate uridyltransferase (GALT), those responsible for sulfate assimilation (S-transporter adenylyltransferase, SAT). addition, carbohydrate sulfotransferase galactose-6-sulfurylase addition removal groups to backbone were evaluated. Structural changes, such as thallus softening, analyzed using Fourier-transformed infrared spectroscopy (FTIR) spectra, ploidy assessed flow cytometry. The results showed downregulation most precursors or charge transformation into hexoses synthesis. Furthermore, alterations FTIR spectrum carrageenan from MEJA-induced fertile thalli, disappearance flattening absorption bands, observed. promptness these events thalli spores seem confirm mitotic production haploid MEJA.